Cytotoxicity comparison of Bio C Sealer against multiple root canal sealers.

Background: The aim of this study was to compare the cytotoxicity of calcium silicate-based endodontic sealer, Bio-C® Sealer, with other calcium silicate-based sealers: BioRoot™ RCS, one silicon-based sealer combined with calcium silicate particles: GuttaFlow® Bioseal, one resin MTA-based root canal sealer: MTA Fillapex®, and an epoxy resin-based sealer: AH Plus®. Material and Methods: NIH 3T3 fibroblasts were cultured and sealers extracts were obtained. Cytotoxicity was evaluated by the MTS assay and the optical densities of the solutions were measured with a microplate reader. This study was designed with one sample for each control group and n=10 for each treatment group of the different sealants. The results were classified according to the degree of cell viability and underwent statistical analysis with the ANOVA test (p<0.05). The samples were examined under an inverted microscope to evaluate the effect of each sealer on fibroblast cell morphology. Results: Cells incubated with GuttaFlow® Bioseal extract showed the highest cell viability without statistically significant differences with the control group. BioRoot™ RCS and Bio-C® Sealer showed moderate (tending to slight) cytotoxicity and both AH Plus® and MTA Fillapex® showed severe cytotoxicity in comparison with the control group (p<0.05). AH Plus® and MTA Fillapex® were not significantly different from one another; nor was BioRoot™ RCS from Bio-C® Sealer. Microscope examination found that fibroblasts in contact with GuttaFlow® Bioseal and Bio-C® Sealer presented the most similar aspects to the control group both in terms of number and shape. Conclusions: Bio-C® Sealer showed moderate (tending to slight) cytotoxicity compared with the control group, GuttaFlow® Bioseal showed no cytotoxicity, BioRoot™ RCS moderate-slight cytotoxicity and AH Plus® and MTA Fillapex® severe cytotoxicity. Key words:Biocompatibility, calcium silicate-based endodontic sealers, cytotoxicity, endodontic sealer.

Dentin Growth after Direct Pulp Capping with the Different Fractions of Plasma Rich in Growth Factors (PRGF) vs. MTA: Experimental Study in Animal Model.

BACKGROUND: This study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. METHODS: twenty-seven upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth), and untreated (NC) (negative control, n = 5). Fluorochrome markers were injected 24 h before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. RESULTS: The higher percentage of dentin growth was observed in the F2 group (B = 63.25%, A = 36.52%), followed by F1 (B = 57.63%, A = 30,12%) and MTA (B = 38.64%, A = 15.74%). The group with lowest percentage of dentin growth was the NC group (B = 29.22%, A = 7.82%). Significant difference (p < 0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. CONCLUSIONS: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation more intensively than MTA and NC.

Use of Platelet-rich Plasma in Endodontic Procedures in Adults: Regeneration or Repair? A Report of 3 Cases with 5 Years of Follow-up.

INTRODUCTION: Regenerative endodontic procedures (REP) are a promising alternative for necrotic immature teeth in adolescents and children, but very little evidence is available on this alternative in long-lasting necrotic teeth with open apices in adults. REPs are designed to replace damaged structures of the pulp-dentin complex, but no regeneration has been obtained in any of the cases described in necrotic immature permanent teeth with apical periodontitis with histologic results. The results are limited to periapical tissue repair with increasing root length, thickening of the root walls, and apical closure in young patients. In this series of cases, we report on the outcomes of the adjuvant use of autologous platelet-rich plasma (PRP) in endodontic therapy in adults by monitoring periapical tissue healing with periodic periapical radiographs and cone-beam computed tomographic scanning. METHODS: Three teeth with apical periodontitis and open apices in 3 different patients from 21 to 35 years-old were evaluated. An REP was performed with the adjuvant use of PRP. RESULTS: At controls, complete disappearance of the radiolucent lesions and the presence of calcified structures forming bridges occupying the pulp lumen were observed but not an ostensible thickening of root walls with a regeneration of pulp-dentin complex. CONCLUSIONS: The repair of periapical tissues with REPs of open apex teeth with apical periodontitis and in nonsurgical endodontic retreatment appears to be feasible in adults, but no regeneration was obtained in any of the present cases. The use of PRP may be a good choice as an autologous matrix because of its stability and induction; it contains growth factors and bioactive molecules like transforming growth factor beta, bone morphogenic proteins, insulinlike growth factors, and angiogenetic growth factors, which stimulate collagen production, angiogenesis, and cell differentiation. Anti-inflammatory and antibacterial properties have also been reported for this preparation, which are involved in all processes of repair.

Knowledge, aptitudes, and preferences in implant dentistry teaching/training among undergraduate dental students at the University of Barcelona

Background: Oral implant rehabilitation should be considered a treatment option for any edentulous patient and Implant Dentistry is currently a discipline taught in the undergraduate formation. The level of knowledge acquired and how the students perceive the quality of training in Implant Dentistry could assess to know if it is necessary to improve the syllabus. Material and Methods: A questionnaire was developed with 11 questions: Basic knowledge (7); Perception of training received (2); Ways in which students would receive training (2) to be responded anonymously and voluntarily for undergraduates students in the Faculty of Dentistry (University of Barcelona, Spain). Results: One hundred and seven students, 76 third year (Group A) and 31 fourth year (Group B) answered the questionnaire. In Group A, 98.68% of students and in Group B 93.54% believed they were poorly informed; 100% of both groups would prefer to receive more training as part of the degree or as postgraduate training through modular courses imparted by experts (A: 71,05%, B: 70,96%) Training through postgraduate programs or training given by private businesses were the least desirable options (A: 42%, B: 64.51%). Questions about basic knowledge acquired received varying responses, which might indicate a certain level of confusion in this area. Conclusions: The undergraduate syllabus must be revised to include sufficient content and training to allow the student to indicate implant-based treatments based on evidence. Students would prefer training to be included in the undergraduate syllabus (AU)

No disponible

Actualización en Endodoncia 2013 / 2013 Update in Endodontics

Los autores revisan los artículos publicados en las revistas científicas más significativas en el ámbito de la endodoncia durante el año 2013, comparándolos entre ellos, con otros anteriores y con los conceptos clásicos de la endodoncia

The authors review the articles published in the most relevant journals concerning endodontics during the last year 2013, making a comparison between them, as well as with other older ones and with classic concepts in endodontics

Interferometric microscopy study of the surface roughness of Portland cement under the action of different irrigants

OBJECTIVES: Some investigations suggested common Portland cement (PC) as a substitute material for MTA for endodontic use; both MTA and PC have a similar composition. The aim of this study was to determine the surface roughness of common PC before and after the exposition to different endodontic irrigating solutions: 10% and 20% citric acid, 17% ethylenediaminetetraacetic (EDTA) and 5% sodium hypochlorite. Study design: Fifty PC samples in the form of cubes were prepared. PC was mixed with distilled water (powder/liquid ratio 3:1 by weight). The samples were immersed for one minute in 10% and 20% citric acid, 17% EDTA and 5% sodium hypochlorite. After gold coating, PC samples were examined using the New View 100 Zygo interferometric microscope. It was used to examine and register the surface roughness and the profile of two different areas of each sample. Analysis of variance (ANOVA) was carried out, and as the requirements were not met, use was made of the Kruskal-Wallis test for analysis of the results obtained, followed by contrasts using Tukey's contrast tests. RESULTS: Sodium hypochlorite at a concentration of 5% significantly reduced the surface roughness of PC, while 20% citric acid significantly increased surface roughness. The other evaluated citric acid concentration (10%) slightly increased the surface roughness of PC, though statistical significance was not reached. EDTA at a concentration of 17% failed to modify PC surface roughness. Irrigation with 5% sodium hypochlorite and 20% citric acid lowered and raised the roughness values, respectively. CONCLUSIONS: The surface texture of PC is modified as the result of treatment with different irrigating solutions commonly used in endodontics, depending on their chemical composition and concentration

Actualización en endodoncia 2012 / 2012 Update in endodontics

Los autores revisan los artículos publicados en las revistas científicas más significativas en el ámbito de la endodoncia durante el año 2012, comparándolos entre ellos, con otros anteriores y con los conceptos clásicos de la endodoncia (AU)

The authors review the articles published in the most relevant journals concerning endodontics during the last year 2012, making a comparison between them, as well as with other older ones and with classic concepts in endodontics (AU)

Interferometric microscopy study of the surface roughness of Portland cement under the action of different irrigants.

OBJECTIVES: Some investigations suggested common Portland cement (PC) as a substitute material for MTA for endodontic use; both MTA and PC have a similar composition. The aim of this study was to determine the surface roughness of common PC before and after the exposition to different endodontic irrigating solutions: 10% and 20% citric acid, 17% ethylenediaminetetraacetic (EDTA) and 5% sodium hypochlorite. STUDY DESIGN: Fifty PC samples in the form of cubes were prepared. PC was mixed with distilled water (powder/liquid ratio 3:1 by weight). The samples were immersed for one minute in 10% and 20% citric acid, 17% EDTA and 5% sodium hypochlorite. After gold coating, PC samples were examined using the New View 100 Zygo interferometric microscope. It was used to examine and register the surface roughness and the profile of two different areas of each sample. Analysis of variance (ANOVA) was carried out, and as the requirements were not met, use was made of the Kruskal-Wallis test for analysis of the results obtained, followed by contrasts using Tukey's contrast tests. RESULTS: Sodium hypochlorite at a concentration of 5% significantly reduced the surface roughness of PC, while 20% citric acid significantly increased surface roughness. The other evaluated citric acid concentration (10%) slightly increased the surface roughness of PC, though statistical significance was not reached. EDTA at a concentration of 17% failed to modify PC surface roughness. Irrigation with 5% sodium hypochlorite and 20% citric acid lowered and raised the roughness values, respectively. CONCLUSIONS: The surface texture of PC is modified as the result of treatment with different irrigating solutions commonly used in endodontics, depending on their chemical composition and concentration.

Actualización en endodoncia 2011 / 2011 Update in endodontics

Los autores revisan los artículos publicados en las revistas científicas más significativas en el ámbito de la endodoncia durante el año 2011, comparándolos entre ellos, con otros anteriores y con los conceptos clásicos de la endodoncia (AU)

The authors review the articles published in the most relevant journals concerning endodontics during the last year 2011, making a comparison between them, as well as with other older ones and with classic concepts in endodontics (AU)